AN IMPROVED TECHNIQUE FOR STAINING RED HALOPHILIC BACTERIA

An Improved Staining Method for Electron Microscopy

A n a q u e o u s so lu t ion of u r a n y l ace t a t e was first e m p l o y e d as a n e l ec t ron s ta in by W a t s o n (1958). W e h a v e f o u n d t h a t this s ta in , a l t h o u g h f r e q u e n t l y effective, is capr ic ious . T h e s ta in m a y no t inc rease con t r a s t of a g iven spec imen , a n d m a y , in o the r ins tances , l eave u n d e s i r a b l e c o n t a m i...

Osmoregulation in Halophilic Bacteria

An improved technique for clearing and staining plant tissues for detection of nematodes.

Numerous procedures have been used to stain and clear nematode-infected plant tissues (7). Staining with acid fuchsin and destaining with lactophenol (4,7) has been the most widely used technique. More rap id means, of clearing and staining roots include autoclaving (1), exposure to saturated chloral hydrate (6), and root macera t ion after staining (3). However, extreme care is required for th...

Isolation of some bacteria for halophilic microbiome of Urmia Lake

Urmia Lake is the largest lake in the Iranian plateau and the second largest Salt Lake in the world. This study was conducted to identify hypersaline bacteria in the lake through the screening with molecular markers. For the molecular study of the bacterial microbiome of the lake, samples were collected from water, sludge and soil of the different parts of the lake during different seasons of 2...

Reporting an Experience: Improving the Feulgen Staining Technique for Better Visualizing of Nucleus

Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in h...